"Analytical Method Development and Investigation of Caffeine Content from Coffea arabica and Anti-Obesity Formulation as per ICH Q2 (R1) Protocol of Validation
作者: Roshan Godage Sanjay Nipanikar Dheeraj Nagore* Machhindra Chavan Dinesh Hase Deshraj Chumbhale Sonal Daware
刊名: Inventi Impact - Pharm Analysis & Quality Assurance, 2019, Vol.2019
来源数据库: Inventi
原始语种摘要: Precise and sophisticated HPTLC and HPLC methods were developed for determination of caffeine from coffee bean extract. HPTLC analysis was performed on silica gel 60 F254 plate using ethyl acetate: methanol: water in the proportion of 10: 1.4: 1 (v/v/v) as mobile phase. A clear band of caffeine was observed at Rf value 0.55 (+0.1). Caffeine shows linearity within the concentration range of 200-600 ppm with regression coefficient of 0.9987, at 254 nm. HPLC analysis was performed by using mobile phase of methanol and water (40:60) with Zorbax SB C-18 column. Quantification was carried out by using PDA detector at 272nm. The method was validated in terms of specificity, precision, linearity, robustness, accuracy, limit of detection (LOD) and limit of quantification (LOQ) as per ICH Q2 (R1)...
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关键词翻译
关键词翻译
  • caffeine 咖啡碱
  • determination 测定
  • HPTLC 高效薄层色谱法
  • sophisticated 复杂
  • acetate 醋酸盐
  • robustness 坚固性
  • precision 精度
  • analysis 分析
  • capsule 胶囊
  • formulation 配方