This study examined the cardioprotective activity of human umbilical cord blood cells (hUCBC) in doxorubicin (Dxr) induced cardiomyopathy in Wistar rats. Wistar rats treated with doxorubicin (50 mg/kg i.p.) revealed cardiac damage that was manifested by the elevation of serum marker enzymes such as lactate dehydrogenase (LDH), aspartate aminotransaminase (AST) and alanine aminotransaminase (ALT). The animals showed significant changes in the biochemical parameter such as in-vivo antioxidant enzyme levels (superoxide dismutase, catalase, glutathione peroxidase and glutathione-S-transferase) and lipid peroxidation levels, lipid profile and histopathological examination. Pretreatment with hUCBC significantly reversed elevation in serum marker enzymes and restored the enzyme activity and... lipid peroxides to near normal levels. Restoration of cellular normality accredits the hUCBC with a cardioprotective role in Dxr-induced cardiac damage.