Objective: To explore the characteristics of post-stroke depressive disorder of acute ischemic stroke using functional magnetic resonance imaging (MRI). (#br)Methods: Twenty patients with post-acute ischemic stroke depressive disorders who were treated in our hospital from August 2014 to August 2016 were enrolled in this study. Twenty healthy individuals during the same period frame for annual physical examination without any diseases were selected as the control group. Both groups received conventional MRI and resting state fMRI (RS-fMRI) scan, images including sagittal T1WI, axial T1WI and rsfMRI images were collected. (#br)Results: Compared with control group, ReHo decreased in bilateral superior frontal gyrus, left inferior temporal gyrus, left inferior parietal lobule and right... cerebellum in the patients group (P < 0.05). In the patients group, the rehos were significantly increased in the middle of the right occipital lobe, cuneus and calcarine sulcus than the normal control group (P < 0.05). (#br)Conclusion: Resting-state Functional magnetic resonance imaging technique is effective to detect the abnormal alteration of brain in patients and is of clinical value for the early diagnosis and treatment of post-acute ischemic stroke depressive disorders.