PCR analysis of genes involved in base excision repair pathway in rice seedlings under Cr(III) exposure
作者: Ying-Chun Huang Na Wang Bi-Yong Huang Chun-Jiao Lu Xiao-Zhang Yu
刊名: Applied Environmental Biotechnology, 2019, Vol.4 (2)
来源数据库: Urban Development Scientific Publishing
DOI: 10.26789/AEB.2019.02.002
关键词: CrDNAGenePCRROSRice seedlings
原始语种摘要: The base excision repair (BER) pathway is an essential defense mechanism in plants against oxidative damage of DNA. Previous studies have reported that chromium (Cr) exposure causes oxidative stress and DNA damage due to accumulation of reactive oxygen species (ROS). In this study, hydroponic experiments were carried out to investigate expression of 21 candidate genes involved in BER pathway in rice seedlings exposed to Cr(III) using qRT-PCR analysis. Changes of H2O2 and O2 content in rice tissues and the relative growth rate of rice seedlings were also determined. Results indicated that Cr(III) exposure caused dose-dependent inhibition on the relative growth rate of rice seedlings. H2O2 content in roots was significantly increased, while changes of H2O2 and O􀀀 2 content in shoots was...
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关键词翻译
关键词翻译
  • exposure 暴露
  • involved 涉及
  • pathway 轨道
  • excision 切除
  • analysis 分析
  • relative 相对的
  • PCR Page Control Register
  • repair 修理
  • investigate 
  • expression 表示