Degenerative lumbar disc disease: in vivo data support the rationale for the selection of appropriate animal models
作者: M FusellierJ ClouetO GauthierM TryfonidouC Le VisageC Guicheux
作者单位: 1ONIRIS Imaging Diagnosis, ONIRIS, Atlanpôle-La Chantrerie, Route de Gachet, Nantes, F-44307, France.
刊名: Eur Cell Mater, 2020, Vol.39 , pp.18-47
来源数据库: European Cells and Materials (eCM)
DOI: 10.22203/eCM.v039a02
原始语种摘要: Appropriate cell sources, bioactive factors and biomaterials for generation of functional and integrated annulus fibrosus (AF) tissue analogues are still an unmet need. In the present study, the AF cell markers, collagen type I, cluster of differentiation 146 (CD146), mohawk (MKX) and smooth muscle protein 22α (SM22α) were found to be suitable indicators of functional AF cell induction. In vitro 2D culture of human AF cells showed that transforming growth factor β1 (TGF-β1) upregulated the expression of the functional AF markers and increased cell contractility, indicating that TGF-β1-pre-treated AF cells were an appropriate cell source for AF tissue regeneration. Furthermore, a tissue engineered construct, composed of polyurethane (PU) scaffold with a TGF-β1-supplemented collagen type I...
全文获取路径: European Cells and Materials (eCM) 
影响因子:4.558 (2012)

  • functional 功能的
  • animal 动物的
  • collagen 胶骨硬蛋白质
  • constructs 建筑图纸生成系统
  • hydrogel 水凝胶
  • organ 
  • tissue 组织
  • regeneration 再生
  • culture 
  • phenotype 表型