Functional cell phenotype induction with TGF-β1 and collagen-polyurethane scaffold for annulus fibrosus rupture repair
作者: J DuR G LongT NakaiD SakaiL M BennekerG ZhouB LiD EglinJ C IatridisM AliniS GradZ Li
作者单位: 1AO Research Institute Davos, Clavadelerstrasse 8, 7270, Davos Platz,
刊名: Eur Cell Mater, 2020, Vol.39 , pp.1-17
来源数据库: European Cells and Materials (eCM)
DOI: 10.22203/eCM.v039a01
原始语种摘要: Appropriate cell sources, bioactive factors and biomaterials for generation of functional and integrated annulus fibrosus (AF) tissue analogues are still an unmet need. In the present study, the AF cell markers, collagen type I, cluster of differentiation 146 (CD146), mohawk (MKX) and smooth muscle protein 22α (SM22α) were found to be suitable indicators of functional AF cell induction. In vitro 2D culture of human AF cells showed that transforming growth factor β1 (TGF-β1) upregulated the expression of the functional AF markers and increased cell contractility, indicating that TGF-β1-pre-treated AF cells were an appropriate cell source for AF tissue regeneration. Furthermore, a tissue engineered construct, composed of polyurethane (PU) scaffold with a TGF-β1-supplemented collagen type I...
全文获取路径: European Cells and Materials (eCM) 
影响因子:4.558 (2012)

  • scaffold 脚手架
  • collagen 胶骨硬蛋白质
  • hydrogel 水凝胶
  • phenotype 表型
  • polyurethane 聚氨酯
  • induction 诱发
  • constructs 建筑图纸生成系统
  • biomaterial 生物材料
  • repair 修理
  • regeneration 再生